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PLoS One. 2016 Feb 12;11(2):e0149275. doi: 10.1371/journal.pone.0149275. eCollection 2016.

Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.

Author information

1
Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
2
Laboratory for Immunological and Molecular Cancer Research, Paracelsus Medical University, Salzburg, Austria.
3
Department of Dermatology, Paracelsus Medical University, Salzburg, Austria.
4
Bühlmann Laboratories AG, Schönenbuch, Switzerland.
5
Department of Neurology, Paracelsus Medical University, Salzburg, Austria.

Abstract

Human blood basophils have recently gained interest in addition to their function as allergic effector cells. Previous work suggests the involvement of innate immune mechanisms in the development and exacerbation of allergic responses, which might be mediated by basophils. We assayed the expression levels of Toll-like receptor (TLR) 1, 2, 4 and 6 on purified basophils from birch pollen-, house dust mite-, and non-allergic individuals. Additionally, we compared cytokine and chemokine secretion upon TLR stimulation in these basophil donor groups. Expression of TLR4 on the basophils of the allergic donor groups was decreased and CXCL8 secretion was elevated upon stimulation of TLR1/2 and TLR2/6 compared to the non-allergic donors. Decreased TLR expression and elevated CXCL8 secretion may represent possible mechanisms for aggravation of allergic symptoms in case of parasitic infection.

PMID:
26870962
PMCID:
PMC4752351
DOI:
10.1371/journal.pone.0149275
[Indexed for MEDLINE]
Free PMC Article

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