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Data Brief. 2015 Dec 18;6:438-44. doi: 10.1016/j.dib.2015.12.024. eCollection 2016 Mar.

Data for the crystal structure of APRIL-BAFF-BAFF heterotrimer.

Author information

1
Proteros Biostructures GmbH, D-82152 Planegg, Germany.
2
EMD Serono Research & Development Institute, Billerica, MA 01821, U.S.A.
3
Merck KGaA, D-64293 Darmstadt, Germany.
4
Merck Serono S.A., Aubonne CH-1170, Switzerland.
5
Department of Biochemistry, University of Lausanne, CH-1066 Epalinges, Switzerland.

Abstract

The TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) modulate B cell function by forming homotrimers and heterotrimers. To determine the structure of a heterotrimer of BAFF and APRIL, these ligands were expressed as a single chain protein in HEK 293 cells, purified by affinity and size exclusion chromatographies, and crystallized. Crystals belonging to the orthorhombic crystal system with a space group of C2221 diffracted to 2.43 Å. Initial structural solution was obtained by the molecular replacement method, and the structure was further refined to an R factor of 0.179 and free R factor of 0.234. The atomic coordinates and structure factors have been deposited into the Protein Data Bank (accession code 4ZCH).

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