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FEBS Lett. 1989 Nov 20;258(1):127-32.

Sequence analysis of the cloned streptococcal cell surface antigen I/II.

Author information

1
Department of Immunology, United Medical School of Guy's Hospital, London, England.

Abstract

The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequences. The N-terminal region (60-550) has alanine-rich repeats and is predicted to be alpha-helical. However, the C-terminal region (800-1540) is proline-rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N-Terminal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N-terminal and the latter the C-terminal portions of the intact antigen.

PMID:
2687020
DOI:
10.1016/0014-5793(89)81632-1
[Indexed for MEDLINE]
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