Format

Send to

Choose Destination
J Cereb Blood Flow Metab. 2016 May;36(5):862-90. doi: 10.1177/0271678X16630991. Epub 2016 Feb 11.

In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use.

Author information

1
Department of Pharmacy, University of Copenhagen, Denmark.
2
Institute of Pharmaceutical Science, King's College London, UK.
3
Klinik und Poliklinik für Anästhesiologie, University of Wurzburg, Germany.
4
Université d'Artois (UArtois), LBHE, EA 2465, Lens, France.
5
Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
6
Institute of Biophysics, Biological Research Centre, HAS, Szeged, Hungary.
7
Institute of Biochemistry, University of Muenster, Germany.
8
Department of Biological Sciences, The Open University, Walton Hall, Milton Keynes, UK.
9
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, WI, USA.
10
Division of Hematology and Medical Oncology, Weill Cornell Medical College, NY, USA.
11
Department of Pharmacy, University of Copenhagen, Denmark birger.brodin@sund.ku.dk.

Abstract

The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This "blood-brain barrier" function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.

KEYWORDS:

Blood–brain barrier; astrocytes; endothelium; pericytes; stem cells

PMID:
26868179
PMCID:
PMC4853841
DOI:
10.1177/0271678X16630991
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center