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Methods Mol Biol. 2016;1398:313-8. doi: 10.1007/978-1-4939-3356-3_24.

Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama-cho 8916-5, Ikoma, Nara, 630-0192, Japan.
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama-cho 8916-5, Ikoma, Nara, 630-0192, Japan. saijo@bs.naist.jp.
3
JST, Precursory Research for Embryonic Science and Technology, 45-3, Yonban-cho 5-3, Chiyoda-ku, Tokyo, 102-8666, Japan. saijo@bs.naist.jp.

Abstract

After Colletotrichum storage methods in Chapter 23 , we describe here experimental methods for the inoculation of Colletotrichum higginsianum (C.h.) on Arabidopsis leaves. We put a particular focus on the methods for lesion measurements after the drop-inoculation of the leaves and on C.h. biomass measurements in the leaves by RT-qPCR analysis. As an option, we also briefly describe methods for counting C.h. entry ratio.

KEYWORDS:

Arabidopsis; Biomass measurements; Colletotrichum higginsianum; Lesion measurements; RT-qPCR

PMID:
26867633
DOI:
10.1007/978-1-4939-3356-3_24
[Indexed for MEDLINE]

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