Format

Send to

Choose Destination
Curr Opin Struct Biol. 2016 Apr;37:145-51. doi: 10.1016/j.sbi.2016.01.014. Epub 2016 Feb 8.

MCM: one ring to rule them all.

Author information

1
The Francis Crick Institute, Clare Hall Laboratory, South Mimms, Herts EN6 3LD, United Kingdom.
2
The Francis Crick Institute, Clare Hall Laboratory, South Mimms, Herts EN6 3LD, United Kingdom. Electronic address: john.diffley@crick.ac.uk.

Abstract

Precise replication of the eukaryotic genome is achieved primarily through strict regulation of the enzyme responsible for DNA unwinding, the replicative helicase. The motor of this helicase is a hexameric AAA+ ATPase called MCM. The loading of MCM onto DNA and its subsequent activation and disassembly are each restricted to separate cell cycle phases; this ensures that a functional replisome is only built once at any replication origin. In recent years, biochemical and structural studies have shown that distinct conformational changes in MCM, each requiring post-translational modifications and/or the activity of other replication proteins, define the various stages of the chromosome replication cycle. Here, we review recent progress in this area.

PMID:
26866665
DOI:
10.1016/j.sbi.2016.01.014
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center