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Pharm Res. 2016 May;33(5):1220-34. doi: 10.1007/s11095-016-1867-7. Epub 2016 Feb 10.

Multimodal Dispersion of Nanoparticles: A Comprehensive Evaluation of Size Distribution with 9 Size Measurement Methods.

Author information

1
Institut Galien Paris-Sud, CNRS, Univ. Paris-Sud, University Paris-Saclay, 92296, Châtenay-Malabry, France.
2
INP - Ecole d'Ingénieurs de PURPAN, Département Sciences Agronomiques & Agroalimentaires, Université de Toulouse, Toulouse, France.
3
INP - Ecole d'Ingénieurs de PURPAN, Laboratoire de Chimie Agro-Industrielle, Université de Toulouse, Toulouse, France.
4
INRA, UMR 1010 CAI, Toulouse, France.
5
Institut Galien Paris-Sud, CNRS, Univ. Paris-Sud, University Paris-Saclay, 92296, Châtenay-Malabry, France. christine.vauthier@u-psud.fr.

Abstract

PURPOSE:

Evaluation of particle size distribution (PSD) of multimodal dispersion of nanoparticles is a difficult task due to inherent limitations of size measurement methods. The present work reports the evaluation of PSD of a dispersion of poly(isobutylcyanoacrylate) nanoparticles decorated with dextran known as multimodal and developed as nanomedecine.

METHODS:

The nine methods used were classified as batch particle i.e. Static Light Scattering (SLS) and Dynamic Light Scattering (DLS), single particle i.e. Electron Microscopy (EM), Atomic Force Microscopy (AFM), Tunable Resistive Pulse Sensing (TRPS) and Nanoparticle Tracking Analysis (NTA) and separative particle i.e. Asymmetrical Flow Field-Flow Fractionation coupled with DLS (AsFlFFF) size measurement methods.

RESULTS:

The multimodal dispersion was identified using AFM, TRPS and NTA and results were consistent with those provided with the method based on a separation step prior to on-line size measurements. None of the light scattering batch methods could reveal the complexity of the PSD of the dispersion.

CONCLUSIONS:

Difference between PSD obtained from all size measurement methods tested suggested that study of the PSD of multimodal dispersion required to analyze samples by at least one of the single size particle measurement method or a method that uses a separation step prior PSD measurement.

KEYWORDS:

light scattering; microscopy; nanoparticle tracking analysis; particle size distribution; tunable resistive pulse sensing

PMID:
26864858
DOI:
10.1007/s11095-016-1867-7
[Indexed for MEDLINE]

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