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Chem Commun (Camb). 2016 Mar 11;52(20):3899-902. doi: 10.1039/c5cc10646j. Epub 2016 Feb 11.

The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongates α1-2-fucosyltransferase.

Author information

1
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.

Abstract

A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.

PMID:
26864394
PMCID:
PMC4775349
DOI:
10.1039/c5cc10646j
[Indexed for MEDLINE]
Free PMC Article

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