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Sci Rep. 2016 Feb 11;6:20937. doi: 10.1038/srep20937.

Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies.

Author information

1
Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
2
Laboratory of Cellular and Structural Biology and Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
3
Department for General and Molecular Botany, Ruhr-University Bochum, Bochum, Germany.

Abstract

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emphasis on the nuclear pore complex, holding great potential for applications in basic science and biotechnology.

PMID:
26864114
PMCID:
PMC4750058
DOI:
10.1038/srep20937
[Indexed for MEDLINE]
Free PMC Article

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