Format

Send to

Choose Destination
J Biol Chem. 2016 Apr 8;291(15):7915-25. doi: 10.1074/jbc.M115.711440. Epub 2016 Feb 9.

Identification and Characterization of IgdE, a Novel IgG-degrading Protease of Streptococcus suis with Unique Specificity for Porcine IgG.

Author information

1
From the Department of Molecular Biology and Umeå Centre for Microbial Research, Umeå University, 90187 Umeå, Sweden.
2
the Institute for Microbiology, Center for Infection Medicine, University of Veterinary Medicine Hannover, 30173 Hannover, Germany, and.
3
the Institute for Microbiology, Center for Infection Medicine, University of Veterinary Medicine Hannover, 30173 Hannover, Germany, and the Institute for Bacteriology und Mycology, Centre for Infectious Diseases, College of Veterinary Medicine, University Leipzig, 04103 Leipzig, Germany.
4
From the Department of Molecular Biology and Umeå Centre for Microbial Research, Umeå University, 90187 Umeå, Sweden, ulrich.von-pawel@molbiol.umu.se.

Abstract

Streptococcus suisis a major endemic pathogen of pigs causing meningitis, arthritis, and other diseases. ZoonoticS. suisinfections are emerging in humans causing similar pathologies as well as severe conditions such as toxic shock-like syndrome. Recently, we discovered an IdeS family protease ofS. suisthat exclusively cleaves porcine IgM and represents the first virulence factor described, linkingS. suisto pigs as their natural host. Here we report the identification and characterization of a novel, unrelated protease ofS. suisthat exclusively targets porcine IgG. This enzyme, designated IgdE forimmunoglobulinG-degradingenzyme ofS. suis, is a cysteine protease distinct from previous characterized streptococcal immunoglobulin degrading proteases of the IdeS family and mediates efficient cleavage of the hinge region of porcine IgG with a high degree of specificity. The findings that allS. suisstrains investigated possess the IgG proteolytic activity and that piglet serum samples contain specific antibodies against IgdE strongly indicate that the protease is expressedin vivoduring infection and represents a novel and putative important bacterial virulence/colonization determinant, and a thus potential therapeutic target.

KEYWORDS:

Streptococcus; antibody; cysteine protease; immunoglobulin G (IgG); proteolytic enzyme; substrate specificity

PMID:
26861873
PMCID:
PMC4824999
DOI:
10.1074/jbc.M115.711440
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center