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Biosens Bioelectron. 2016 Jun 15;80:359-365. doi: 10.1016/j.bios.2016.02.002. Epub 2016 Feb 2.

Giant magnetoresistive sensor array for sensitive and specific multiplexed food allergen detection.

Author information

1
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. Electronic address: elaineng@stanford.edu.
2
Department of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Otolaryngology, Stanford University, Stanford, CA 94305, USA.
3
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA.

Abstract

Current common allergen detection methods, including enzyme-linked immunosorbent assays (ELISAs) and dip-stick methods, do not provide adequate levels of sensitivity and specificity for at-risk allergic patients. A method for performing highly sensitive and specific detection of multiple food allergens is thus imperative as food allergies are becoming increasingly recognized as a major healthcare concern, affecting an estimated 4% of the total population. We demonstrate first instance of sensitive and specific multiplexed detection of major peanut allergens Ara h 1 and Ara h 2, and wheat allergen Gliadin using giant magnetoresistive (GMR) sensor arrays. Commercialized ELISA kits for Ara h 1 and Ara h 2 report limits of detection (LODs) at 31.5 ng/mL and 0.2 ng/mL, respectively. In addition, the 96-well-based ELISA developed in-house for Gliadin was found to have a LOD of 40 ng/mL. Our multiplexed GMR-based assay demonstrates the ability to perform all three assays on the same chip specifically and with sensitivities at LODs about an order of magnitude lower than those of 96-well-based ELISAs. LODs of GMR-based assays developed for Ara h 1, Ara h 2, and Gliadin were 7.0 ng/mL, 0.2 ng/mL, and 1.5 ng/mL, respectively, with little to no cross-reactivity. These LODs are clinically important as some patients could react strongly against such low allergen levels. Given the limitations of current industrial detection technology, multiplexed GMR-based assays provide a method for highly sensitive and specific simultaneous detection of any combination of food-product allergens, thus protecting allergic patients from life-threatening events, including anaphylaxis, by unintentional consumption.

KEYWORDS:

Food allergens; Giant magnetoresistance; Magnetic-based immunoassays; Multiplexed immunoassays; Sensor arrays

PMID:
26859787
DOI:
10.1016/j.bios.2016.02.002
[Indexed for MEDLINE]

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