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Anal Chem. 2016 Mar 15;88(6):3348-53. doi: 10.1021/acs.analchem.5b04939. Epub 2016 Feb 22.

Highly Selective Detection of 5-Methylcytosine in Genomic DNA Based on Asymmetric PCR and Specific DNA Damaging Reagents.

Author information

1
College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
2
Zhongnan Hospital, Wuhan University , Wuhan, Hubei 430071, P. R. China.
3
School of Chemistry and Environmental Engineering, Wuhan Institute of Technology , Wuhan, Hubei 430073, P. R. China.

Abstract

DNA methylation is a significant epigenetic modification of the genome that is involved in regulating many cellular processes. An increasing number of human diseases have been discovered to be associated with aberrant DNA methylation, and aberrant DNA methylation has been deemed to be a potential biomarker for diseases such as cancers. A safe, nontoxic, and sensitive method for accurate detection of 5-methylcytosine in genomic DNA is extremely useful for early diagnosis and therapy of cancers. In this paper, we established a novel system to detect 5-methylcytosine, which is based on bisulfite treatment, asymmetric PCR, and specific DNA damaging reagents. Our method could be used for identifying the loci of 5mC in genomic DNA and detecting the DNA methylation levels in tissues as well.

PMID:
26859062
DOI:
10.1021/acs.analchem.5b04939
[Indexed for MEDLINE]

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