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Cell Med. 2015 Aug 26;8(1-2):3-7. doi: 10.3727/215517915X689100. eCollection 2015 Dec 17.

Cryopreservation of Adipose-Derived Mesenchymal Stem Cells.

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Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
† Okayama Saidaiji Hospital , Okayama , Japan.
‡ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Niigata , Japan.
§ Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
¶ Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
# Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.


Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. However, many studies have reported that cellular activity after freezing and thawing may be affected by the solutions used for cryopreservation. Dimethyl sulfoxide (DMSO) is commonly used as a cryopreservation medium as it diffuses into the cell through the plasma membrane and protects the cells from the damage caused by freezing. As substitutes for DMSO or animal-derived serum, cell banker series, polyvinylpyrrolidone (PVP), sericin and maltose, and methyl cellulose (MC) have been investigated for their clinical applications. It is critical to develop a reliable cell cryopreservation protocol for regenerative medicine using MSCs.


Adipose-derived mesenchymal stem cells (ASCs); Cell banker series; Cryopreservation; Dimethyl sulfoxide (DMSO); Polyvinylpyrrolidone (PVP)

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