Expression of microRNA-133 inhibits epithelial-mesenchymal transition in lung cancer cells by directly targeting FOXQ1
Arch Bronconeumol. 2016 Oct;52(10):505-11.
doi: 10.1016/j.arbres.2015.10.016.
Epub 2016 Feb 5.
[Article in
English,
Spanish]
Affiliations
- 1 School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China; Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, China.
- 2 School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China.
- 3 School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China. Electronic address: jichenggreen@163.com.
Abstract
Introduction:
MicroRNA (miR) was implicated in the tumorigenesis of many types of cancer, but no study was conducted on the exact role of miR-133 in lung cancer.
Methods:
We have identified miR-133 as a putative regulator of FOXQ1 expression, and investigated the potential involvement of miR-133 in the migration and invasion of lung cancer cells, as well as the underlying molecular mechanism.
Results:
MiR-133 directly targeted and down-regulated FOXQ1 expression, which in turn reduced TGF-β level. MiR-133 was down-regulated in lung cancer cell lines A549 and HCC827, and its re-expression significantly inhibited the migration and invasion of the lung cancer cells. Further investigation revealed that this inhibition was caused by reversing the epithelial-mesenchymal transition, evidenced by miR-133 induced elevation of epithelial marker E-cadherin, and reduction of mesenchymal marker Vimentin.
Conclusions:
Our study is the first to identify miR-133 as a biomarker for lung cancer. It functions to down-regulate FOXQ1, and inhibit epithelial-mesenchymal transition, which antagonizes lung cancer tumorigenesis. Therefore our data support the role of miR-133 as a potential molecular therapeutic tool in treating lung cancer.
Keywords:
Cáncer de pulmón; Epithelial-mesenchymal transition; FOXQ1; Lung cancer; MicroARN-133; MicroRNA-133; TGF-β; Transición epitelio-mesenquimatosa.
Copyright © 2015 SEPAR. Publicado por Elsevier España, S.L.U. All rights reserved.
MeSH terms
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3' Untranslated Regions
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Antigens, CD
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Cadherins / biosynthesis
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Cadherins / genetics
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Cell Adhesion
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Cell Line, Tumor
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Epithelial-Mesenchymal Transition / physiology*
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Forkhead Transcription Factors / biosynthesis*
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Forkhead Transcription Factors / genetics
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Gene Expression Regulation, Neoplastic*
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology*
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MicroRNAs / genetics
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MicroRNAs / physiology*
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Neoplasm Invasiveness
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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RNA, Neoplasm / genetics
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RNA, Neoplasm / physiology*
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Transforming Growth Factor beta / biosynthesis
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Transforming Growth Factor beta / genetics
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Vimentin / biosynthesis
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Vimentin / genetics
Substances
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3' Untranslated Regions
-
Antigens, CD
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CDH1 protein, human
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Cadherins
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FOXQ1 protein, human
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Forkhead Transcription Factors
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MIRN133 microRNA, human
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MicroRNAs
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Neoplasm Proteins
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RNA, Neoplasm
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Transforming Growth Factor beta
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Vimentin