Novel Structures and Temporal Changes of Arginine Catabolic Mobile Elements in Methicillin-Resistant Staphylococcus aureus Genotypes ST5-MRSA-II and ST764-MRSA-II in Japan

Noriko Urushibara; Mitsuyo Kawaguchiya; Mayumi Onishi; Keiji Mise; Meiji Soe Aung; Nobumichi Kobayashi

doi:10.1128/AAC.02356-15

Novel Structures and Temporal Changes of Arginine Catabolic Mobile Elements in Methicillin-Resistant Staphylococcus aureus Genotypes ST5-MRSA-II and ST764-MRSA-II in Japan

Antimicrob Agents Chemother. 2016 Apr 22;60(5):3119-22. doi: 10.1128/AAC.02356-15. Print 2016 May.

Authors

Noriko Urushibara¹, Mitsuyo Kawaguchiya², Mayumi Onishi², Keiji Mise³, Meiji Soe Aung², Nobumichi Kobayashi²

Affiliations

¹ Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan noriko-u@sapmed.ac.jp.
² Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
³ Department of Admissions, Center for Medical Education, Sapporo Medical University, Sapporo, Japan.

Abstract

Twenty-two of 1,103 methicillin-resistant Staphylococcus aureus (MRSA) isolates containing the type II staphylococcal cassette chromosome mec element (SCCmec) (collected in Hokkaido, Japan, from 2008 to 2011) harbored the arginine catabolic mobile element (ACME). Five genetic variations were identified in the ACME-staphylococcal cassette chromosome mec composite islands, 66 to 79 kb in size. The percentage of ACME carriage temporally increased from 0.85% to 4.5% in parallel with the emergence of shorter variants (66 to 72 kb). Shorter variants may have a selective advantage and accelerate the dissemination of ACME in Japanese MRSA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Chromosomes, Bacterial / genetics
DNA, Bacterial / genetics
Genotype
Japan
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics*

Substances

Anti-Bacterial Agents
DNA, Bacterial

Grants and funding

This study was supported by a Grant-in-Aid for Scientific Research (grant number 24590751) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT).