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Sci Rep. 2016 Feb 9;6:20316. doi: 10.1038/srep20316.

Dictyocaulus viviparus genome, variome and transcriptome elucidate lungworm biology and support future intervention.

Author information

1
The McDonnell Genome Institute, Washington University in St Louis, MO 63108, USA.
2
Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover 30559, Germany.
3
HHMI, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
4
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
5
Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.

Abstract

The bovine lungworm, Dictyocaulus viviparus (order Strongylida), is an important parasite of livestock that causes substantial economic and production losses worldwide. Here we report the draft genome, variome, and developmental transcriptome of D. viviparus. The genome (161 Mb) is smaller than those of related bursate nematodes and encodes fewer proteins (14,171 total). In the first genome-wide assessment of genomic variation in any parasitic nematode, we found a high degree of sequence variability in proteins predicted to be involved host-parasite interactions. Next, we used extensive RNA sequence data to track gene transcription across the life cycle of D. viviparus, and identified genes that might be important in nematode development and parasitism. Finally, we predicted genes that could be vital in host-parasite interactions, genes that could serve as drug targets, and putative RNAi effectors with a view to developing functional genomic tools. This extensive, well-curated dataset should provide a basis for developing new anthelmintics, vaccines, and improved diagnostic tests and serve as a platform for future investigations of drug resistance and epidemiology of the bovine lungworm and related nematodes.

PMID:
26856411
PMCID:
PMC4746573
DOI:
10.1038/srep20316
[Indexed for MEDLINE]
Free PMC Article

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