Format

Send to

Choose Destination
J Mol Biol. 2016 Mar 27;428(6):1304-1314. doi: 10.1016/j.jmb.2016.01.032. Epub 2016 Feb 6.

Interactions between the Microtubule Binding Protein EB1 and F-Actin.

Author information

1
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, 46556, USA.
2
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, 55455, USA.
3
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, 60637, USA.
4
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, 46556, USA. Electronic address: hgoodson@nd.edu.

Abstract

Many cellular processes including cell division and cell migration require coordination between the actin and microtubule (MT) cytoskeletons. This coordination is as-yet poorly understood, but proteins such as formins and IQGAP1 are known to be involved. We show that the MT binding protein EB1 (end-binding protein 1), a key regulator of MT dynamics, can bind directly to filamentous actin (F-actin) F-actin. We determined that the EB1:F-actin interaction is salt sensitive and weak under physiological salt concentrations but might be relevant in contexts where the local concentration of actin is high. Using bioinformatics and mutagenesis, we found that the EB1:F-actin binding site partially overlaps the well-characterized EB1:MT binding interface. Congruently, competition experiments indicate that EB1 can bind to F-actin or MTs but not both simultaneously. These observations suggest that EB1:F-actin interactions may negatively regulate EB1:MT interactions, and we speculate that this interaction may assist cells in differentially regulating MT stability in the actin-rich cortex as opposed to the cell interior.

KEYWORDS:

EB1; actin; cytoskeleton; microtubules; plus-end tracking protein

PMID:
26854759
PMCID:
PMC4834876
DOI:
10.1016/j.jmb.2016.01.032
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center