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J Invest Dermatol. 2016 May;136(5):1012-21. doi: 10.1016/j.jid.2015.11.032. Epub 2016 Feb 15.

Oral Supplementation with Cocoa Extract Reduces UVB-Induced Wrinkles in Hairless Mouse Skin.

Author information

1
WCU Biomodulation Major, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea.
2
CHA Cancer Institute, CHA University, Seongnam, Republic of Korea.
3
CHA Cancer Institute, CHA University, Seongnam, Republic of Korea; Department of Biochemistry, School of Medicine, CHA University, Seongnam, Republic of Korea.
4
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea; Institute of Human-Environment Interface Biology, Seoul National University, Seoul, Republic of Korea; Department of Dermatology, Seoul National University Boramae Hospital, Seoul, Republic of Korea.
5
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea; Institute of Human-Environment Interface Biology, Seoul National University, Seoul, Republic of Korea.
6
Health Foods Team, Lotte R&D Center, Seoul, Republic of Korea.
7
CHAUM Life Center, School of Medicine, CHA University, Seoul, Republic of Korea.
8
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea; Institute of Human-Environment Interface Biology, Seoul National University, Seoul, Republic of Korea; Institute on Aging, Seoul National University, Seoul, Republic of Korea.
9
CHA Cancer Institute, CHA University, Seongnam, Republic of Korea; Department of Biochemistry, School of Medicine, CHA University, Seongnam, Republic of Korea. Electronic address: takim@cha.ac.kr.
10
WCU Biomodulation Major, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea; Institute on Aging, Seoul National University, Seoul, Republic of Korea. Electronic address: kiwon@snu.ac.kr.

Abstract

Cacao beans contain various bioactive phytochemicals that could modify the pathogeneses of certain diseases. Here, we report that oral administration of cacao powder (CP) attenuates UVB-induced skin wrinkling by the regulation of genes involved in dermal matrix production and maintenance. Transcriptome analysis revealed that 788 genes are down- or upregulated in the CP supplemented group, compared with the UVB-irradiated mouse skin controls. Among the differentially expressed genes, cathepsin G and serpin B6c play important roles in UVB-induced skin wrinkle formation. Gene regulatory network analysis also identified several candidate regulators responsible for the protective effects of CP supplementation against UVB-induced skin damage. CP also elicited antiwrinkle effects via inhibition of UVB-induced matrix metalloproteinases-1 expression in both the human skin equivalent model and human dermal fibroblasts. Inhibition of UVB-induced activator protein-1 via CP supplementation is likely to affect the expression of matrix metalloproteinases-1. CP supplementation also downregulates the expression of cathepsin G in human dermal fibroblasts. 5-(3',4'-Dihydroxyphenyl)-γ-valerolactone, a major in vivo metabolite of CP, showed effects similar to CP supplementation. These results suggest that cacao extract may offer a protective effect against photoaging by inhibiting the breakdown of dermal matrix, which leads to an overall reduction in wrinkle formation.

PMID:
26854493
DOI:
10.1016/j.jid.2015.11.032
[Indexed for MEDLINE]
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