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Biosci Biotechnol Biochem. 2016;80(4):706-11. doi: 10.1080/09168451.2015.1123604. Epub 2016 Feb 8.

Effect of miR-143-3p on C2C12 myoblast differentiation.

Author information

1
a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
2
b Department of Animal Science , Xichang College , Xichang , China.
3
c Department of Agricultural, Food and Nutritional Science , University of Alberta , Edmonton , Canada.
4
d Sichuan Provincial Agricultural Department, Sichuan Province General Station of Animal Husbandry , Chengdu , China.

Abstract

MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3' untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.

KEYWORDS:

C2C12; Wnt pathway; miR-143-3p; myoblast differentiation

PMID:
26854366
DOI:
10.1080/09168451.2015.1123604
[Indexed for MEDLINE]

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