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Fungal Genet Biol. 2016 Mar;88:54-8. doi: 10.1016/j.fgb.2016.02.001. Epub 2016 Feb 4.

High frame-rate resolution of cell division during Candida albicans filamentation.

Author information

1
Manchester Fungal Infection Group, Institute of Inflammation and Repair, University of Manchester, CTF Building, Grafton Street, Manchester M13 9NT, UK.
2
Department of Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.
3
School of Medicine, Medical Sciences & Nutrition, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK. Electronic address: a.brand@aberdeen.ac.uk.

Abstract

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

KEYWORDS:

Hypha; Nuclear division; Septum

PMID:
26854071
PMCID:
PMC4767323
DOI:
10.1016/j.fgb.2016.02.001
[Indexed for MEDLINE]
Free PMC Article

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