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Genome. 2016 Mar;59(3):173-83. doi: 10.1139/gen-2015-0115. Epub 2015 Dec 18.

Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato.

Author information

1
a Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea.
2
b Department of Plant Science, Gangneung-Wonju National University, Gangneung 210-720, Republic of Korea.
3
c Department of Horticulture, Sunchun National University, Sunchun 540-950, Republic of Korea.
4
d Department of Bioresources Engineering, Sejong University, Seoul 143-747, Republic of Korea.
5
e Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea.

Abstract

Fusarium crown and root rot is a severe fungal disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In this study, the genomic location of the FORL-resistance locus was determined using a set of molecular markers on chromosome 9 and an F2 population derived from FORL-resistant inbred 'AV107-4' (Solanum lycopersicum) × susceptible 'L3708' (Solanum pimpinellifolium). Bioassay performed using Korean FORL strain KACC 40031 showed single dominant inheritance of FORL resistance in the F2 population. In all, 13 polymerase chain reaction-based markers encompassing approximately 3.6-72.0 Mb of chromosome 9 were developed based on the Tomato-EXPEN 2000 map and SolCAP Tomato single nucleotide polymorphism array analysis. These markers were genotyped on 345 F2 plants, and the FORL-resistance locus was found to be present on a pericentromeric region of suppressed chromosomal recombination in chromosome 9. The location of the FORL-resistance locus was further confirmed by testing these markers against diverse commercial tomato and stock cultivars resistant to FORL. A restriction fragment length polymorphism marker, PNU-D4, located at approximately 6.1 Mb of chromosome 9 showed the highest match with the resistance locus and was used for conducting high-resolution melting analysis for marker-assisted selection of FORL resistance.

KEYWORDS:

Fusarium crown and root rot; Fusarium oxysporum f. sp. radicis-lycopersici; Solanum lycopersicum; disease resistance; marqueur moléculaire; molecular marker; pourriture fusarienne de la tige et des racines; résistance à la maladie

PMID:
26853773
DOI:
10.1139/gen-2015-0115
[Indexed for MEDLINE]

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