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Sci Rep. 2016 Feb 8;6:20724. doi: 10.1038/srep20724.

Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.

Huang W1,2,3, Yao Y1,2,3, Wang S1,2,3, Xia Y1,2,3, Yang X1,2,3, Long Q1,2,3, Sun W1,2,3, Liu C1,2,3, Li Y1,2,3, Chu X1,2,3, Bai H1,2,3, Yao Y1,2,3, Ma Y1,2,3.

Author information

1
Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.
2
Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.
3
Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.

Abstract

A. baumannii infections are becoming more and more serious health issues with rapid emerging of multidrug and extremely drug resistant strains, and therefore, there is an urgent need for the development of nonantibiotic-based intervention strategies. This study aimed at identifying whether an outer membrane protein with molecular weight of about 22 kDa (Omp22) holds the potentials to be an efficient vaccine candidate and combat A. baumannii infection. Omp22 which has a molecule length of 217 amino acids kept more than 95% conservation in totally 851 reported A. baumannii strains. Recombinant Omp22 efficiently elicited high titers of specific IgG in mice. Both active and passive immunizations of Omp22 increased the survival rates of mice, suppressed the bacterial burdens in the organs and peripheral blood, and reduced the levels of serum inflammatory cytokines and chemokines. Opsonophagocytosis assays showed in vitro that Omp22 antiserum had highly efficient bactericidal activities on clonally distinct clinical A. baumannii isolates, which were partly complements-dependent and opsonophagocytic killing effects. Additionally, administration with as high as 500 μg of Omp22 didn't cause obvious pathological changes in mice. In conclusion, Omp22 is a novel conserved and probably safe antigen for developing effective vaccines or antisera to control A. baumannii infections.

PMID:
26853590
PMCID:
PMC4745112
DOI:
10.1038/srep20724
[Indexed for MEDLINE]
Free PMC Article

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