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J Agric Food Chem. 2016 Feb 24;64(7):1540-8. doi: 10.1021/acs.jafc.5b05931. Epub 2016 Feb 15.

Anti-inflammatory Cerebrosides from Cultivated Cordyceps militaris.

Chiu CP1, Liu SC2, Tang CH2, Chan Y3, El-Shazly M4, Lee CL5, Du YC1, Wu TY6, Chang FR1,7,8,9, Wu YC10,11,12,13.

Author information

1
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University , Kaohsiung 807, Taiwan.
2
Graduate Institute of Basic Medical Science, China Medical University , Taichung 404, Taiwan.
3
Institute of Microbiology, Chung Shan Medical University , Taichung 402, Taiwan.
4
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University , Organization of African Unity Street, Abassia, Cairo 11566, Egypt.
5
Department of Cosmeceutics, China Medical University , Taichung 404, Taiwan.
6
Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 404, Taiwan.
7
Cancer Center, Kaohsiung Medical University Hospital , Kaohsiung 80708, Taiwan.
8
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
9
Department of Marine Biotechnology and Resources, National Sun Yat-sen University , Kaohsiung 80424, Taiwan.
10
School of Pharmacy, College of Pharmacy, China Medical University , Taichung 40402, Taiwan.
11
Research Center for Chinese Herbal Medicine, China Medical University , Taichung 40402, Taiwan.
12
Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40402, Taiwan.
13
Center of Molecular Medicine, China Medical University Hospital , Taichung 40402, Taiwan.

Abstract

Cordyceps militaris (bei-chong-chaw, northern worm grass) is a precious and edible entomopathogenic fungus, which is widely used in traditional Chinese medicine (TCM) as a general booster for the nervous system, metabolism, and immunity. Saccharides, nucleosides, mannitol, and sterols were isolated from this fungus. The biological activity of C. militaris was attributed to the saccharide and nucleoside contents. In this study, the aqueous methanolic fraction of C. militaris fruiting bodies exhibited a significant anti-inflammatory activity. Bioactivity-guided fractionation of the active fraction led to the isolation of eight compounds, including one new and two known cerebrosides (ceramide derivatives), two nucleosides, and three sterols. Cordycerebroside A (1), the new cerebroside, along with soyacerebroside I (2) and glucocerebroside (3) inhibited the accumulation of pro-inflammatory iNOS protein and reduced the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophages. This is the first study on the isolation of cerebrosides with anti-inflammatory activity from this TCM.

KEYWORDS:

Cordyceps militaris; LPS-stimulated RAW264.7 macrophages; anti-inflammatory; cerebrosides; fungal fruiting bodies

PMID:
26853111
DOI:
10.1021/acs.jafc.5b05931
[Indexed for MEDLINE]

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