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Biosens Bioelectron. 2017 Mar 15;89(Pt 1):606-611. doi: 10.1016/j.bios.2016.01.076. Epub 2016 Jan 29.

Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips.

Author information

1
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France; Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
2
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
3
SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Gipuzkoa, Spain.
4
Graphenea S.A., Tolosa Hiribidea, 76, 20018 Donostia, San Sebastian, Spain.
5
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
6
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France. Electronic address: sabine.szunerits@iri.univ-lille1.fr.

Abstract

Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the analytical approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixtures onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quantitative clinical analysis.

KEYWORDS:

Biomarker; Folate receptor; Folic acid; Folic acid protein; Graphene; SPR

PMID:
26852830
DOI:
10.1016/j.bios.2016.01.076
[Indexed for MEDLINE]

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