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Biosens Bioelectron. 2016 Jun 15;80:265-272. doi: 10.1016/j.bios.2016.01.070. Epub 2016 Jan 29.

Practical, highly sensitive, and regenerable evanescent-wave biosensor for detection of Hg(2+) and Pb(2+) in water.

Author information

1
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China.
2
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China. Electronic address: hemiao@mail.tsinghua.edu.cn.
3
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
4
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.

Abstract

A "turn-on" evanescent-wave fiber biosensor based on functional nucleic acids was developed for on-site detection of Hg(2+) and Pb(2+) in environmental water samples. A two-step strategy was conducted to take advantage of the rapid binding of T-T mismatches with Hg(2+) and the cleavage of a substrate by 8-17 DNAzyme with Pb(2+) in solution, as well as sensitive DNA detection on a liquid-solid interface based on evanescent wave. The biosensor demonstrated high sensitivity (with a detection limit of 22 pM for Hg(2+) and 20 nM for Pb(2+)) and selectivity without any signal amplification, rapid detection (within 13 min per test), low cost (10-20 Chinese yuan per sample), and multiple-cycle regeneration (at least 18 times). The method was also successfully applied on Hg(2+) and Pb(2+) detection in real environmental water samples.

KEYWORDS:

Fluorescent biosensor; Functional nucleic acids; Heavy metal ions; On-site; Regeneration

PMID:
26851585
DOI:
10.1016/j.bios.2016.01.070
[Indexed for MEDLINE]

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