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FEMS Yeast Res. 2016 Mar;16(2):fow010. doi: 10.1093/femsyr/fow010. Epub 2016 Feb 5.

Absence of Rtt109p, a fungal-specific histone acetyltransferase, results in improved acetic acid tolerance of Saccharomyces cerevisiae.

Author information

1
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
2
State Key Laboratory of Microbial Metabolism, Shanghai 200240, China School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China xqzhao@sjtu.edu.cn.
3
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China State Key Laboratory of Microbial Metabolism, Shanghai 200240, China School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Abstract

RTT109 is a histone acetyltransferase for the acetylation of histone H3. It is still not clear whether RTT109 plays a role in regulation of gene expression under environmental stresses. In this study, the involvement of RTT109 in acetic acid stress tolerance of Saccharomyces cerevisiae was investigated. It was revealed that the absence of RTT109 enhanced resistance to 5.5 g L(-1) acetic acid, which was indicated by improved growth of RTT109Δ mutant compared with that of the wild-type BY4741 strain. Meanwhile, the lag phase was shortened for 48 h and glucose consumption completed 36 h in advance for RTT109Δ mutant compared to the wild-type strain, with ethanol production rate increased from 0.39 to 0.60 g L(-1) h(-1). Significantly, elevated transcription levels of HSP12, CTT1 and GSH1, as well as increased activities of antioxidant enzymes were observed in RTT109Δ under acetic acid stress. Improved flocculation of RTT109Δ compared to that of the control strain BY4741 under the acetic acid stress was also observed. These results suggest that the absence of RTT109 not only activates transcription of stress responsive genes, but also improves resistance to oxidative stress, which ultimately contributes to improved acetic acid tolerance in S. cerevisiae.

KEYWORDS:

RTT109; Saccharomyces cerevisiae; acetic acid tolerance; ethanol production; histone modification; oxidative stress

PMID:
26851403
DOI:
10.1093/femsyr/fow010
[Indexed for MEDLINE]

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