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Environ Toxicol Pharmacol. 2016 Mar;42:146-55. doi: 10.1016/j.etap.2016.01.013. Epub 2016 Jan 21.

Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells.

Author information

1
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China.
2
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Saybagh District Animal Husbandry and Veterinary Station, Urumqi 830000, Xinjiang, China.
3
Jiaozuo Entry-Exit Inspection and Quarantine Bureau of P.R. China, Jiaozuo 454001, Henan, China.
4
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China. Electronic address: jcbian@yzu.edu.cn.

Abstract

The aim of this study was to investigate the effects of ZEA on the cytoskeletal structure, and factors specifically expressed by Sertoli cells. Primary Sertoli cells from rats aged 18-21 days were exposed to increasing ZEA concentrations (0, 5, 10, 20 μg mL(-1)) for 24 h. The results of immunofluorescence showed disruption of α-tubulin filaments and F-actin bundles, and damage to the nucleus of Sertoli cells on exposure to ZEA. In the control group, the protein level expression of androgen-binding protein (ABP), transferrin, vimentin, N-cadherin, and follicle-stimulating hormone receptor (FSHR) were decreased significantly (p<0.05, p<0.01). The mRNA levels of ABP, transferrin, vimentin, N-cadherin, and FSHR varied significantly in the experimental group (p<0.05). The results of enzyme-linked immunosorbent assay indicated a significant decrease in the levels of inhibin-β and transferrin in the cultural supernatants (p<0.05). Additionally, the ultrastructural analysis indicated the absence of mitochondria and Golgi apparatus, and presence of vacuoles in the cytoplasm. These findings showed that ZEA treatment can damage the cytoskeletal structure and affect specific secretory functions of Sertoli cells, which may be an underlying cause of ZEA-induced reproductive toxicity.

KEYWORDS:

Cytoskeletal structure; Secretory function; Sertoli cells; Zearalenone

PMID:
26851377
DOI:
10.1016/j.etap.2016.01.013
[Indexed for MEDLINE]

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