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Anticancer Res. 2016 Feb;36(2):721-30.

Hypoxia-regulated MicroRNAs in Gastroesophageal Cancer.

Author information

1
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark mette@oncology.au.dk.
2
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark.
3
Department of Pathology, Aarhus University Hospital, Aarhus, Denmark.
4
Department of Oncology, Rigshospitalet, Copenhagen, Denmark.
5
Department of Oncology, Odense University Hospital, Odense, Denmark.
6
Department of Oncology, Aarhus University Hospital, Aarhus, Denmark.

Abstract

BACKGROUND/AIM:

The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC).

MATERIALS AND METHODS:

microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays and validated by quantitative real-time polymerase chain reaction. Candidate HRMs were measured in pre-therapeutic tumor samples from 195 patients with GEC.

RESULTS:

Expression of miR-210 was shown to be significantly induced in esophageal squamous cell carcinoma (9.26-fold, p<0.001) and adenocarcinoma cell lines (4.95-fold, p<0.001) and miR-27a-star was significantly up-regulated in adenocarcinoma cell lines (4.79-fold, p=0.04). A weak but significant correlation between miR-210 expression and a 15-gene hypoxia signature was observed (Pearson r correlation: r=0.38, p<0.001). No significant associations of HRMs and clinical outcome in patients with GEC were identified.

CONCLUSION:

This study supports the involvement of hypoxia on miRNAs in vitro and confirms the role of miR-210 as being a universal HRM.

KEYWORDS:

Gastroesophageal cancer; hypoxia; miRNA-210; microRNAs

PMID:
26851030
[Indexed for MEDLINE]

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