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Physiol Genomics. 2016 Apr;48(4):320-4. doi: 10.1152/physiolgenomics.00124.2015. Epub 2016 Feb 5.

MicroRNA expression profiling in skeletal muscle reveals different regulatory patterns in high and low responders to resistance training.

Author information

1
Department of Life and Materials Engineering, Nagoya Institute of Technology, Nagoya, Japan;
2
Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan;
3
Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan; and.
4
Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan; and Department of Sports Medicine for Health Promotion, Tokyo Medical University, Tokyo, Japan.
5
Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan; and safujita@fc.ritsumei.ac.jp.

Abstract

Large variability exists in muscle adaptive response to resistance exercise (RE) training between individuals. Recent studies have revealed a significant role for microRNAs (miRNAs) in skeletal muscle plasticity. In this study, we investigated how RE affects miRNA expression and whether the variability of muscle hypertrophy to RE training may be attributed to differential miRNA regulation in the skeletal muscle. To screen high and low responders to RE, we had 18 young men perform arm curl exercise training. After screening, all the men performed 12 wk of lower body RE training, but only the high or low responders participated in the acute RE test before training. Muscle biopsies were obtained from the vastus lateralis muscle at baseline, 3 h after acute RE, and after the training period. Total RNA was extracted from the skeletal muscle, and miRNA expression (800 miRNAs) was analyzed. RE training increased the cross-sectional area of the biceps brachii (-1.7-26.1%), quadriceps (2.2-16.8%), and hamstrings (1.6-18.4%). Eighty-five and 102 miRNAs were differentially expressed after acute and chronic RE, respectively (P < 0.05). Seventeen miRNAs, especially 23b-3p, 26a-5p, 32-5p, 148b-3p, and 376a-3p, were differentially expressed at baseline, and 23 miRNAs, especially let-7a-5p, 95, 148a-3p, and 376a-3p, and 26 miRNAs, especially 30d-5p and 376a-3p, were differentially regulated after acute and chronic RE, respectively, in the skeletal muscle between high and low responders, indicating that the expression patterns of several miRNAs are altered by acute or chronic RE, and that miRNAs are involved in skeletal muscle adaptation to RE training.

KEYWORDS:

comprehensive expression analysis; exercise; muscle hypertrophy

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