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Gene. 1989 Oct 15;82(1):77-82.

The catalytic core of the sunY intron of bacteriophage T4.

Author information

1
Department of Biological Sciences, State University of New York, Albany 12222.

Abstract

The self-splicing sunY intron of bacteriophage T4 shares a common secondary structure with other group I introns. A long open reading frame within the intron is entirely 3' of the structural elements conserved in all group I introns. This catalytic core is the smallest yet described for a self-splicing intron. An internal deletion of 728 nucleotides (nt), leaving 196 nt at the 5' end and 109 nt at the 3' end, allows normal self-splicing. Transcripts terminating 196 nt 3' of the 5' splice site retain catalytic activity.

PMID:
2684777
DOI:
10.1016/0378-1119(89)90032-2
[Indexed for MEDLINE]

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