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Biochem Biophys Res Commun. 2016 Feb 26;471(1):57-62. doi: 10.1016/j.bbrc.2016.01.181. Epub 2016 Feb 2.

The role of stabilization centers in protein thermal stability.

Author information

1
Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok krt 2, H-1117 Budapest, Hungary.
2
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India.
3
Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok krt 2, H-1117 Budapest, Hungary. Electronic address: simon.istvan@ttk.mta.hu.

Abstract

The definition of stabilization centers was introduced almost two decades ago. They are centers of noncovalent long range interaction clusters, believed to have a role in maintaining the three-dimensional structure of proteins by preventing their decay due to their cooperative long range interactions. Here, this hypothesis is investigated from the viewpoint of thermal stability for the first time, using a large protein thermodynamics database. The positions of amino acids belonging to stabilization centers are correlated with available experimental thermodynamic data on protein thermal stability. Our analysis suggests that stabilization centers, especially solvent exposed ones, do contribute to the thermal stabilization of proteins.

KEYWORDS:

Protein stability; Stability changing mutations; Stabilization centers; Thermal stability; Thermophiles

PMID:
26845354
DOI:
10.1016/j.bbrc.2016.01.181
[Indexed for MEDLINE]
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