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EBioMedicine. 2015 Nov 11;2(12):2080-6. doi: 10.1016/j.ebiom.2015.11.020. eCollection 2015 Dec.

Hepatitis E Virus Superinfection and Clinical Progression in Hepatitis B Patients.

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Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany.
Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany; Department of Molecular Biology, Tran Hung Dao Hospital, Hanoi, Viet Nam.
Institute of Clinical Infectious Diseases, Tran Hung Dao Hospital, Hanoi, Viet Nam.
Department of Pathophysiology, Vietnam Military Medical University, Ha Dong, Hanoi, Viet Nam.
Department of Biotechnology, Technical University of Berlin, Berlin, Germany.


Hepatitis E virus (HEV) infection may cause acute hepatitis and lead to hepatic failure in developing and developed countries. We studied HEV seroprevalences in patients with hepatitis B virus (HBV) infection to understand the consequences of HEV superinfection in a Vietnamese population. This cross-sectional study was conducted from 2012 to 2013 and included 1318 Vietnamese patients with HBV-related liver diseases and 340 healthy controls. The case group included patients with acute (n = 26) and chronic hepatitis B (n = 744), liver cirrhosis (n = 160), hepatocellular carcinoma (n = 166) and patients with both liver cirrhosis and hepatocellular carcinoma (n = 222). Anti-HEV IgG and IgM antibodies were assessed in patients and controls by ELISA. HEV-RNA was identified by PCR assays and sequencing. Seroprevalences of anti-HEV IgG among hepatitis B patients and controls were 45% and 31%, respectively (adjusted P = 0.034). Anti-HEV IgM seroprevalences were 11.6% and 4.7% in patients and controls, respectively (adjusted P = 0.005). Seroprevalences were higher among the elder individuals. When stratifying for patient groups, those with liver cirrhosis had the highest anti-HEV IgG (52%) and anti-HEV IgM (19%) seroprevalences. Hepatitis B patients with current HEV infection had abnormal liver function tests compared to patients with past or without HEV infection. One HEV isolate was retrieved from a patient with both liver cirrhosis and hepatocellular carcinoma and identified as HEV genotype 3. This study indicates high prevalences of HEV infection in Vietnamese HBV patients and among healthy individuals and shows that HEV superinfection may influence the outcome and progression of HBV-related liver disease.


AFP, alpha-feto protein; AHB, acute hepatitis B; ALT, alanine amino transferase; AST, aspartate amino transferase; CHB, chronic hepatitis B; HBV infection; HBV, hepatitis B virus; HBV-related liver diseases; HCC, hepatocellular carcinoma; HEV seroprevalence; HEV superinfection; HEV, hepatitis E virus; Hepatitis E virus; IgG, immunoglobulin G; IgM, immunoglobulin M; LC, liver cirrhosis; ORF, open reading frame; PLT, platelets; RBC, red blood cells; WBC, white blood cells

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