Format

Send to

Choose Destination
Nat Commun. 2016 Feb 4;7:10584. doi: 10.1038/ncomms10584.

GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex.

Author information

1
Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.
2
Centre for Neuroscience, Imperial College Faculty of Medicine, Hammersmith Hospital, Imperial College London, London W12 0NN, UK.
3
Department of Clinical and Experimental Epilepsy, Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.
4
Centre for Neural Circuits and Behaviour, University of Oxford, Oxford OX1 3SR, UK.

Abstract

GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find that Nkx2-1-derived interneurons possess functional synaptic connections before emerging pyramidal cell networks. Subsequent interneuron circuit maturation is both subtype and layer dependent. Glutamatergic input onto fast spiking (FS), but not somatostatin-positive, non-FS interneurons increases over development. Interneurons of both subtype located in layers (L) 4 and 5b engage in transient circuits that disappear after the somatosensory critical period. These include a pathway mediated by L5b somatostatin-positive interneurons that specifically targets L4 during the first postnatal week. The innervation patterns of immature cortical interneuron circuits are thus neither static nor progressively strengthened but follow a layer-specific choreography of transient connections that differ from those of the adult brain.

PMID:
26843463
PMCID:
PMC4743032
DOI:
10.1038/ncomms10584
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center