Format

Send to

Choose Destination
Methods Mol Biol. 2016;1392:63-9. doi: 10.1007/978-1-4939-3360-0_7.

High-Resolution Melt Curve Analysis in Cancer Mutation Screen.

Author information

1
Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Unit 72, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
2
Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Unit 72, 1515 Holcombe Blvd, Houston, TX, 77030, USA. kppatel@mdanderson.org.

Abstract

High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.

KEYWORDS:

Double-stranded DNA binding fluorescent dyes; High-resolution melt curve analysis; PCR

PMID:
26843047
DOI:
10.1007/978-1-4939-3360-0_7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center