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Plant Cell. 2016 Feb;28(2):345-66. doi: 10.1105/tpc.15.00910. Epub 2016 Feb 3.

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis.

Author information

1
School of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom ubech@essex.ac.uk.
2
Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom.
3
School of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom.
4
College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, United Kingdom.
5
Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
6
School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
7
Department of Statistics, University of Warwick, Coventry CV4 7AL, United Kingdom.
8
Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.

Abstract

In Arabidopsis thaliana, changes in metabolism and gene expression drive increased drought tolerance and initiate diverse drought avoidance and escape responses. To address regulatory processes that link these responses, we set out to identify genes that govern early responses to drought. To do this, a high-resolution time series transcriptomics data set was produced, coupled with detailed physiological and metabolic analyses of plants subjected to a slow transition from well-watered to drought conditions. A total of 1815 drought-responsive differentially expressed genes were identified. The early changes in gene expression coincided with a drop in carbon assimilation, and only in the late stages with an increase in foliar abscisic acid content. To identify gene regulatory networks (GRNs) mediating the transition between the early and late stages of drought, we used Bayesian network modeling of differentially expressed transcription factor (TF) genes. This approach identified AGAMOUS-LIKE22 (AGL22), as key hub gene in a TF GRN. It has previously been shown that AGL22 is involved in the transition from vegetative state to flowering but here we show that AGL22 expression influences steady state photosynthetic rates and lifetime water use. This suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses.

PMID:
26842464
PMCID:
PMC4790877
DOI:
10.1105/tpc.15.00910
[Indexed for MEDLINE]
Free PMC Article

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