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Biotechniques. 2016 Feb 1;60(2):91-4. doi: 10.2144/000114381. eCollection 2016 Feb.

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries.

Author information

1
School of Pharmacy, The University of Maryland, Baltimore, MD.
2
School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia.

Abstract

Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure-function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution that is well within the capability of most molecular biologists, but tools for the design and analysis of such experiments are limited. Here we introduce a suite of freely available online tools to make the construction and analysis of chimeric libraries readily accessible to the novice. REcut (http://qpmf.rx.umaryland.edu/REcut.html) facilitates the choice of DNA fragmentation strategy, while Xover (http://qpmf.rx.umaryland.edu/Xover.html) analyzes chimeric mutants to reveal recombination patterns and extract quantitative data.

KEYWORDS:

DNA shuffling; PCR; SCHEMA; Staggered Extension Process; artificial evolution; chimera; crossover; directed evolution; mosaic; mutant library; recombination; restriction enzyme

PMID:
26842355
DOI:
10.2144/000114381
[Indexed for MEDLINE]
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