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Biotechniques. 2016 Feb 1;60(2):91-4. doi: 10.2144/000114381. eCollection 2016 Feb.

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries.

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School of Pharmacy, The University of Maryland, Baltimore, MD.
School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Australia.


Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure-function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution that is well within the capability of most molecular biologists, but tools for the design and analysis of such experiments are limited. Here we introduce a suite of freely available online tools to make the construction and analysis of chimeric libraries readily accessible to the novice. REcut ( facilitates the choice of DNA fragmentation strategy, while Xover ( analyzes chimeric mutants to reveal recombination patterns and extract quantitative data.


DNA shuffling; PCR; SCHEMA; Staggered Extension Process; artificial evolution; chimera; crossover; directed evolution; mosaic; mutant library; recombination; restriction enzyme

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