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J Negat Results Biomed. 2016 Feb 3;15:2. doi: 10.1186/s12952-016-0045-0.

ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model.

Author information

1
Key Laboratory of Brain Functional Genomics (East China Normal University), Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics (East China Normal University), School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China. minliu920120@163.com.
2
Key Laboratory of Brain Functional Genomics (East China Normal University), Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics (East China Normal University), School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China. tianmissing@sina.com.
3
Key Laboratory of Brain Functional Genomics (East China Normal University), Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics (East China Normal University), School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China. fangjing@126.com.
4
Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, 227 South Chongqing Road, Shanghai, 20025, China. suyindang@shsmu.edu.cn.
5
Key Laboratory of Brain Functional Genomics (East China Normal University), Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics (East China Normal University), School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China. wzhang@sat.ecnu.edu.cn.

Abstract

BACKGROUND:

In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene.

RESULTS:

We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control.

CONCLUSIONS:

We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

PMID:
26841794
PMCID:
PMC4739331
DOI:
10.1186/s12952-016-0045-0
[Indexed for MEDLINE]
Free PMC Article

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