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Int J Biol Macromol. 2016 May;86:468-80. doi: 10.1016/j.ijbiomac.2016.01.110. Epub 2016 Feb 1.

Development of glycan specific lectin based immunoassay for detection of prostate specific antigen.

Author information

1
Laboratory of Native Proteins, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India; Department of Medical Biotechnology, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, India.
2
Laboratory of Native Proteins, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India; Department of Biochemistry, National Institute for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai 400012, India. Electronic address: sham83badgujar@gmail.com.
3
Laboratory of Native Proteins, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India.
4
Department of Radiation Oncology, Tata Memorial Centre, Mumbai 400012, India.
5
Laboratory of Membrane Biology, National Centre for Cell Science, Savitribai Phule Pune University Campus, Pune 411007, India.
6
Department of Medical Biotechnology, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, India.

Abstract

We describe an analytical approach for the detection and verification of glycosylation patterns of prostate specific antigen (PSA), a key biomarker currently used for understanding the onset and prognosis of prostate cancer. PSA has been purified from the human seminal plasma and total PSA from prostate cancer sera. PSA is a monomeric glycoprotein with an apparent molecular mass 28040.467 Da, which exhibits a characteristic protease activity against casein and gelatin. Its optimal protease activity is centered on neutral pH. Peptide mass fingerprint analysis of the purified PSA has yielded peptides that partially match with known database sequences (Uniprot ID P07288). Tryptic digestion profile of isolated PSA, infer the exclusive nature of PSA and may be additive molecule in the dictionary of seminal proteins. Surface plasmon resonance and lectin immunoassay revealed direct interaction between a newly developed anti-PSA monoclonal antibody (C4E6) and PSA. A lectin based immunoassay is reported here which was achieved with the C4E6 anti-PSA antibody and biotinylated plant lectins. This investigation provides an alternative method to isolate and quantify PSA with altered glycosylation which might be seen in the prostate cancer and developing a lectin based immunoassay to detect PSA in serum of prostate cancer patients.

KEYWORDS:

Biomarkers; Glycosylation; Lectin-based immunoassay; Prostate cancer; Prostate specific antigen

PMID:
26840176
DOI:
10.1016/j.ijbiomac.2016.01.110
[Indexed for MEDLINE]

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