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Sci Rep. 2016 Feb 1;6:20086. doi: 10.1038/srep20086.

Streptomyces thermoautotrophicus does not fix nitrogen.

Author information

1
Harvard Medical School, 200 Longwood Ave, Boston MA 02130.
2
Wyss Institute, 3 Blackfan Cir, Boston MA 02115.
3
Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla S2125ZAA, Santa Fe, Argentina.
4
Dept. Life Sciences, Imperial College, London, SW7 2AZ, UK.
5
Department of Plant Biology, Michigan State University, 612 Wilson Rd, East Lansing, MI USA 48824.
6
Institute for Biology I, BioSC, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany.
7
TheLab Inc, 1340 E. 6th Street Suite 603, Los Angeles, CA USA 90021.

Abstract

Streptomyces thermoautotrophicus UBT1 has been described as a moderately thermophilic chemolithoautotroph with a novel nitrogenase enzyme that is oxygen-insensitive. We have cultured the UBT1 strain, and have isolated two new strains (H1 and P1-2) of very similar phenotypic and genetic characters. These strains show minimal growth on ammonium-free media, and fail to incorporate isotopically labeled N2 gas into biomass in multiple independent assays. The sdn genes previously published as the putative nitrogenase of S. thermoautotrophicus have little similarity to anything found in draft genome sequences, published here, for strains H1 and UBT1, but share >99% nucleotide identity with genes from Hydrogenibacillus schlegelii, a draft genome for which is also presented here. H. schlegelii similarly lacks nitrogenase genes and is a non-diazotroph. We propose reclassification of the species containing strains UBT1, H1, and P1-2 as a non-Streptomycete, non-diazotrophic, facultative chemolithoautotroph and conclude that the existence of the previously proposed oxygen-tolerant nitrogenase is extremely unlikely.

PMID:
26833023
PMCID:
PMC4735515
DOI:
10.1038/srep20086
[Indexed for MEDLINE]
Free PMC Article

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