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Amino Acids. 2016 May;48(5):1161-71. doi: 10.1007/s00726-016-2181-8. Epub 2016 Feb 1.

Amino acids in the cultivation of mammalian cells.

Author information

1
Institute of Pharmaceutical Chemistry, University of Marburg, 35032, Marburg, Germany. andrew.salazar@external.merckgroup.com.
2
Biopharm Materials & Technologies R&D, Merck Lifescience, 64293, Darmstadt, Germany. andrew.salazar@external.merckgroup.com.
3
Institute of Pharmaceutical Chemistry, University of Marburg, 35032, Marburg, Germany.
4
Biopharm Materials & Technologies R&D, Merck Lifescience, 64293, Darmstadt, Germany.

Abstract

Amino acids are crucial for the cultivation of mammalian cells. This importance of amino acids was realized soon after the development of the first cell lines, and a solution of a mixture of amino acids has been supplied to cultured cells ever since. The importance of amino acids is further pronounced in chemically defined mammalian cell culture media, making the consideration of their biological and chemical properties necessary. Amino acids concentrations have been traditionally adjusted to their cellular consumption rates. However, since changes in the metabolic equilibrium of amino acids can be caused by changes in extracellular concentrations, metabolomics in conjunction with flux balance analysis is being used in the development of culture media. The study of amino acid transporters is also gaining importance since they control the intracellular concentrations of these molecules and are influenced by conditions in cell culture media. A better understanding of the solubility, stability, dissolution kinetics, and interactions of these molecules is needed for an exploitation of these properties in the development of dry powdered chemically defined media for mammalian cells. Due to the complexity of these mixtures however, this has proven to be challenging. Studying amino acids in mammalian cell culture media will help provide a better understanding of how mammalian cells in culture interact with their environment. It would also provide insight into the chemical behavior of these molecules in solutions of complex mixtures, which is important in the understanding of the contribution of individual amino acids to protein structure.

KEYWORDS:

Amino acid; Bioprocess; Biotechnology; Cell culture media; Media formulation

PMID:
26832172
PMCID:
PMC4833841
DOI:
10.1007/s00726-016-2181-8
[Indexed for MEDLINE]
Free PMC Article

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