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Arch Microbiol. 2016 May;198(4):347-52. doi: 10.1007/s00203-016-1193-9. Epub 2016 Feb 1.

Bacillus nitroreducens sp. nov., a humus-reducing bacterium isolated from a compost.

Author information

1
College of Food Science and Technology, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China.
2
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, China.
3
Fuzhou Arboretum, Fuzhou, 350012, China.
4
College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
5
College of Food Science and Technology, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China. yzhao@shou.edu.cn.
6
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, China. lzhuang@soil.gd.cn.

Abstract

A Gram-staining-positive, facultative anaerobic, motile and rod-shaped bacterium, designated GSS08(T), was isolated from a windrow compost pile and characterized by means of a polyphasic approach. Growth occurred with 0-4 % (w/v) NaCl (optimum 1 %), at pH 6.5-9.5 (optimum pH 7.5) and at 20-45 °C (optimum 37 °C). Anaerobic growth occurred with anthraquinone-2,6-disulphonate, fumarate and NO3 (-) as electron acceptor. The main respiratory quinone was MK-7. The predominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>5 %) were iso-C15:0 (43.1 %), anteiso-C15:0 (27.4 %) and iso-C16:0 (8.3 %). The DNA G + C content was 39.6 mol%. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSS08(T) formed a phyletic lineage with the type strain of Bacillus humi DSM 16318(T) with a high sequence similarity of 97.5 %, but it displayed low sequence similarity with other valid species in the genus Bacillus (<96.0 %). The DNA-DNA relatedness between strains GSS08(T) and B. humi DSM 16318(T) was 50.8 %. The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain GSS08(T) represents a novel species, for which the name Bacillus nitroreducens sp. nov. is proposed. The type strain is GSS08(T) (=KCTC 33699(T) = MCCC 1K01091(T)).

KEYWORDS:

Bacillus nitroreducens sp. nov.; Phylogenetic analysis; Polyphasic taxonomy

PMID:
26832132
DOI:
10.1007/s00203-016-1193-9
[Indexed for MEDLINE]

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