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Saudi J Gastroenterol. 2016 Jan-Feb;22(1):30-6. doi: 10.4103/1319-3767.173756.

MicroRNA-155-enhanced autophagy in human gastric epithelial cell in response to Helicobacter pylori.

Author information

1
Department of Gastroenterology, 309 Hospital of Chinese Peoples Liberation Army, Beijing, China.

Abstract

BACKGROUND/AIM:

MicroRNAs (miRNAs) are a class of small noncoding RNAs acting as posttranscriptional gene expression regulators in many physiological and pathological conditions. MiR-155 is one kind of miRNAs that plays an important role in causing various diseases. However, the precise molecular mechanism of the ectopic expression of miR-155 in Helicobacter pylori infection remains poorly understood. Autophagy has recently been identified as an effective way to control the intracellular bacterium survival. In the present study, we demonstrate a novel role of miR-155 in regulating the autophagy-mediated anti-H. pylori response.

PATIENTS AND METHODS:

Totally 86 H. pylori-positive patients together with 10 H. pylori-negative, healthy control subjects were included in the study. Correlation between immunohistochemical grades and miR-155 expression were determined. Molecular mechanism of miR-155 on regulation of autophagy and elimination of intracellular H. pylori were determined using the GES-1 cell model.

RESULTS:

We found that overexpression of miR-155 by transfecting miR-155 mimics could significantly decrease the survival of intracellular H. pylori, and this process was through induction of autophagy. Furthermore, there was a significant correlation between miR-155 and immunohistochemical grades in H. pylori-positive patients, and miR-155 expression were decreased in the intestinal metaplasia group.

CONCLUSIONS:

The results have indicated that the miR-155 expression level plays a key role in immunity response against H. pylori and this might provide potential targets for the future treatment of H. pylori-related diseases.

PMID:
26831604
PMCID:
PMC4763526
DOI:
10.4103/1319-3767.173756
[Indexed for MEDLINE]
Free PMC Article

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