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Biol Res. 2016 Feb 1;49:7. doi: 10.1186/s40659-016-0067-6.

Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model.

Author information

1
Laboratorio de Neurobiología Molecular y Celular, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Delegación Tlalpan, 14269, Mexico, D.F., Mexico. marisol.orozco.ibarra@gmail.com.
2
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, Colonia La Fama, Delegación Tlalpan, 14269, Mexico D.F., Mexico. jorgemen89@hotmail.com.
3
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, Colonia La Fama, Delegación Tlalpan, 14269, Mexico D.F., Mexico. anneniv@gmail.com.
4
Laboratorio de Neuroinmunología y Neuro-oncología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Delegación Tlalpan, 14269, Mexico D.F., Mexico. benpio76@hotmail.com.
5
Laboratorio de Neuroinmunología y Neuro-oncología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Delegación Tlalpan, 14269, Mexico D.F., Mexico. roxytbp@yahoo.com.mx.
6
Departamento de Ciencias Naturales, CNI, Universidad Autónoma Metropolitana Cuajimalpa, Av. Vasco de Quiroga 4871: Col. Santa Fe, Delegación Cuajimalpa de Morelos, 05348, Mexico D.F., Mexico. evazquezc@gmail.com.
7
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, Colonia La Fama, Delegación Tlalpan, 14269, Mexico D.F., Mexico. maldonado.perla@gmail.com.
8
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Mexico D.F., Mexico. pedraza@unam.mx.
9
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, Colonia La Fama, Delegación Tlalpan, 14269, Mexico D.F., Mexico. echanezc@gmail.com.

Abstract

BACKGROUND:

Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-1α) and up-regulation of HIF-1α-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells.

RESULTS:

We found that CoCl2 induced the stabilization of HIF-1α and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1α stabilization, activity not previously reported for AGE and SAC.

CONCLUSIONS:

Obtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1α and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions.

PMID:
26830333
PMCID:
PMC4736283
DOI:
10.1186/s40659-016-0067-6
[Indexed for MEDLINE]
Free PMC Article

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