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J Mol Biol. 2016 May 8;428(9 Pt B):1822-32. doi: 10.1016/j.jmb.2016.01.024. Epub 2016 Jan 30.

The MCM Helicase Motor of the Eukaryotic Replisome.

Author information

1
Architecture and Dynamics of Macromolecular Machines, Clare Hall Laboratory, The Francis Crick Institute, Blanche Lane, South Mimms EN6 3LD, United Kingdom.
2
Architecture and Dynamics of Macromolecular Machines, Clare Hall Laboratory, The Francis Crick Institute, Blanche Lane, South Mimms EN6 3LD, United Kingdom. Electronic address: alessandro.costa@crick.ac.uk.

Abstract

The MCM motor of the CMG helicase powers ahead of the eukaryotic replication machinery to unwind DNA, in a process that requires ATP hydrolysis. The reconstitution of DNA replication in vitro has established the succession of events that lead to replication origin activation by the MCM and recent studies have started to elucidate the structural basis of duplex DNA unwinding. Despite the exciting progress, how the MCM translocates on DNA remains a matter of debate.

KEYWORDS:

AAA+ ATPase; DNA replication; MCM helicase; molecular motor; replication origin activation

PMID:
26829220
DOI:
10.1016/j.jmb.2016.01.024
[Indexed for MEDLINE]

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