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Am J Pathol. 2016 Apr;186(4):722-32. doi: 10.1016/j.ajpath.2015.10.033. Epub 2016 Jan 30.

Biological Processes Discovered by High-Throughput Sequencing.

Author information

1
Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia.
2
Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia; Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia. Electronic address: ad8q@virginia.edu.

Abstract

Advances in DNA and RNA sequencing technologies have completely transformed the field of genomics. High-throughput sequencing (HTS) is now a widely used and accessible technology that allows scientists to sequence an entire transcriptome or genome in a timely and cost-effective manner. Application of HTS techniques has led to many key discoveries, including the identification of long noncoding RNAs, microDNAs, a family of small extrachromosomal circular DNA species, and tRNA-derived fragments, which are a group of small non-miRNAs that are derived from tRNAs. Furthermore, public sequencing repositories provide unique opportunities for laboratories to parse large sequencing databases to identify proteins and noncoding RNAs at a scale that was not possible a decade ago. Herein, we review how HTS has led to the discovery of novel nucleic acid species and uncovered new biological processes during the course.

PMID:
26828742
PMCID:
PMC5807928
DOI:
10.1016/j.ajpath.2015.10.033
[Indexed for MEDLINE]
Free PMC Article

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