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Nat Methods. 2016 Mar;13(3):248-50. doi: 10.1038/nmeth.3737. Epub 2016 Feb 1.

TruSPAdes: barcode assembly of TruSeq synthetic long reads.

Author information

1
Center for Algorithmic Biotechnology, Institute for Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.
2
Department of Computer Science and Engineering, University of California at San Diego, La Jolla, California, USA.

Abstract

The recently introduced TruSeq synthetic long read (TSLR) technology generates long and accurate virtual reads from an assembly of barcoded pools of short reads. The TSLR method provides an attractive alternative to existing sequencing platforms that generate long but inaccurate reads. We describe the truSPAdes algorithm (http://bioinf.spbau.ru/spades) for TSLR assembly and show that it results in a dramatic improvement in the quality of metagenomics assemblies.

PMID:
26828418
DOI:
10.1038/nmeth.3737
[Indexed for MEDLINE]

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