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Vet Q. 2016 Jun;36(2):56-62. doi: 10.1080/01652176.2016.1141257. Epub 2016 Feb 1.

Evaluation of intra-articular injection of autologous platelet lysate (PL) in horses with osteoarthritis of the distal interphalangeal joint.

Author information

1
a Equine Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece.
2
b Surgery and Obstetrics Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece.
3
c Anaesthesiology and Intensive Care Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece.
4
d Department of Biochemistry, Medical School , Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece.

Abstract

BACKGROUND:

Regenerative medicine has become one of the most promising therapies of equine osteoarthritis. Platelet lysate (PL) is rich in bioactive proteins and growth factors that play a crucial role in tissue healing.

OBJECTIVE:

To evaluate the efficacy of intra-articularly injected autologous PL in equine athletes with naturally occurring osteoarthritis.

ANIMALS AND METHODS:

Fifteen warmblood geldings aged 8-19 years with osteoarthritis of the distal interphalangeal joint were included in this study. They were randomly divided into two groups; 10 horses received intra-articular injections of PL and 5 of normal saline (controls). Before treatment, platelet-derived growth factor (PDGF) levels in basal plasma and prepared PL were estimated. Each joint was injected twice within a three-week period. Lameness was evaluated using the American Association of Equine Practitioners grading system, before treatment and 10 days after each intra-articular injection. Horses were examined fortnightly for one year. Radiographic examination was performed six months post-treatment. The generalized estimating equation test was used for statistical analysis.

RESULTS:

Acceptable levels of PDGF were detected in PLs (mean ± SD: 258.0 ± 52.3 pg/ml). The majority of horses (9/10) responded positively to PL treatment presenting lower lameness grades (p < 0.0005) compared to controls 10 days after the second injection, and returned to normal athletic activity. Radiographs revealed no changes in osteoarthritis lesions six months after treatment. One year post-injections, however, all horses relapsed to their initial degree of lameness.

CONCLUSION:

Intra-articularly injected autologous PL is an efficient method for temporarily managing osteoarthritis of the distal interphalangeal joint in athletic horses.

KEYWORDS:

Horse; autologous platelet lysate; distal interphalangeal joint; equine; osteoarthritis; platelet-derived growth factor

PMID:
26828234
DOI:
10.1080/01652176.2016.1141257
[Indexed for MEDLINE]

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