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Biosens Bioelectron. 2016 Jun 15;80:140-145. doi: 10.1016/j.bios.2016.01.052. Epub 2016 Jan 21.

Partially reduced graphene oxide as highly efficient DNA nanoprobe.

Author information

1
Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China.
2
Department of Laboratory Medicine, Second Hospital of Fuzhou, Fuzhou 350007, China.
3
Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic address: 13600885383@139.com.
4
Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, China. Electronic address: chenandhu@163.com.

Abstract

This work investigates the effect of reduction degree on graphene oxide (GO)-DNA interaction and the fluorescence quenching mechanism. Partial reduced graphene oxide (pRGO), which maintains well water-dispersibility, is synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room temperature. The fluorescence quenching enhances with the restoration degree of sp(2) carbon bonds and follows the static quenching mechanism. The binding constant values imply that pRGO has much stronger affinity with ssDNA than GO. Utilizing this highly efficient nanoprobe, a universal sensing strategy is proposed for homogeneous detection of DNA. Compared with the reported GO-based DNA, this present strategy has obvious advantages such as requirement of low nanoprobe dosage, significantly reduced background, fast fluorescence quenching, and improved sensitivity. Even without any amplification process, the limit of detection can reach as low as 50 pM.

KEYWORDS:

DNA; Fluorescence; Partially reduced graphene oxide; Quenching; Sensor

PMID:
26826548
DOI:
10.1016/j.bios.2016.01.052
[Indexed for MEDLINE]

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