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Elife. 2016 Jan 29;5:e07383. doi: 10.7554/eLife.07383.

Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.

Author information

1
System Biosciences, LLC, Mountain View, United States.
2
Transgenic Research Center, Stanford University School of Medicine, Stanford, United States.
3
University of Virginia, Charlottesville, United States.
4
University of Cambridge, Cambridge, United Kingdom.

Abstract

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from "Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET" by Peinado and colleagues, published in Nature Medicine in 2012 (Peinado et al., 2012). The key experiments being replicated are from Figures 4E, as well as Supplementary Figures 1C and 5A. In these experiments, Peinado and colleagues show tumor exosomes enhance metastasis to bones and lungs, which is diminished by reducing Met expression in exosomes (Peinado et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.

KEYWORDS:

MET; Reproducibility Project: Cancer Biology; exosomes; human biology; medicine; metastasis; methodology; mouse; receptor tyrosine kinase

PMID:
26826285
PMCID:
PMC4749561
DOI:
10.7554/eLife.07383
[Indexed for MEDLINE]
Free PMC Article

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