Correlation of interactions between NOS3 polymorphisms and oxygen therapy with retinopathy of prematurity susceptibility

Int J Clin Exp Pathol. 2015 Nov 1;8(11):15250-4. eCollection 2015.

Abstract

Aim: This study was aimed to detect the correlation of nitric oxide synthase 3 (NOS3) gene polymorphisms (T-786C and G894T) and retinopathy of prematurity (ROP) susceptibility. Interaction between NOS3 gene polymorphisms and the duration of oxygen therapy was also explored in ROP babies.

Methods: Genotypes of NOS3 gene polymorphisms were genotyped by MassArray method. Hardy-Weinberg equilibrium (HWE) was used to calculate the representativeness of the cases and controls. Crossover analysis was utilized to explore the gene environment interactions. Relative risk of ROP was presented by odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs).

Results: Among the subject features, oxygen therapy had obvious difference between case and control groups (P<0.05). There existed significant association between-786C allele and ROP susceptibility (P=0.049, OR=0.669, 95% CI=0.447-0.999). Genotypes of T-786C polymorphism and genotypes and alleles of G894T polymorphism did not related to the susceptibility of ROP. Interactions were existed between NOS3 gene polymorphisms and oxygen therapy duration. When the duration of oxygen therapy was less than 17 days, both -786CC genotype and 894GT genotype were correlated with ROP susceptibility (P=0.020, OR=0.115, 95% CI=0.014-0.960; P=0.011, OR=0.294, 95% CI=0.100-0.784).

Conclusion: -786C allele might have a protective effect for ROP. Interactions of -786CC and 894GT genotype with oxygen therapy duration (less than 17 days) were both protection factors of ROP.

Keywords: NOS3 gene; ROP; interaction; oxygen therapy; polymorphisms.

MeSH terms

  • Case-Control Studies
  • Chi-Square Distribution
  • Female
  • Gene Frequency
  • Gene-Environment Interaction*
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Heterozygote
  • Homozygote
  • Humans
  • Infant, Newborn
  • Male
  • Nitric Oxide Synthase Type III / genetics*
  • Odds Ratio
  • Oxygen Inhalation Therapy / adverse effects*
  • Phenotype
  • Polymorphism, Genetic*
  • Protective Factors
  • Retinopathy of Prematurity / diagnosis
  • Retinopathy of Prematurity / enzymology
  • Retinopathy of Prematurity / genetics*
  • Risk Assessment
  • Risk Factors
  • Time Factors

Substances

  • NOS3 protein, human
  • Nitric Oxide Synthase Type III